Abstract: By using Total Electron Content (TEC) at Wuhan station from 1997 to 2007, based on continuous wavelet, cross wavelet and wavelet coherence transform methods, the periodic variation of TEC at this station, the characteristic of TEC association with solar activities and geomagnetic activities are analyzed. Analysis results show that long-term variations of TEC at Wuhan station are mainly controlled by the variations of solar activities. In local time field, several remarkable components including 128～256 days, 256～512 days and 512～1024 days time-scale periodicities exist in TEC daily variation and correspond to the periodic characteristics of sunspot number and the geomagnetic Dst index closely during the same period of time. The average time delay between variations of the TEC and sunspot number is about 1/6 period in 512～1024 days time-scale. TEC at Wuhan station and geomagnetic Dst index are anti-phasic in semiannual time-scale, but this response only exists during high solar activity, further specific mechanism needed to be analyzed in the future.