Influence of impurity on surface morphology and growth kinetics of a lysozyme crystal
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摘要: 采用相差显微镜研究了两种杂质蛋白(荧光标记的溶菌酶和商品溶菌酶中的杂质)对四方溶菌酶晶体表面形貌、{110}和{101}表面生长速度以及{110}面台阶生长动力学的影响. 杂质会影响晶体表面二维生长岛的形貌,并显著降低晶体{110}和{101}面法向生长速度. 杂质会显著降低{110}表面台阶的生长速度, 且对〈110〉方向速度的抑制效果显著强于〈001〉方向. 根据切向台阶速度和过饱和度的关系, 计算得到了台阶动力学系数β和台阶的有效表面能及棱边自由能.Abstract: The influences of impurity on surface morphology and growth kinetics of tetragonal lysozyme crystals were studied. There was a significant difference in morphology of 2D islands on a {110} face when the impurities existed. Impurities significantly decreased the normal growth rates of both the {110} and {101} face. F-lysozyme decreased the normal growth rate of both the {110} face and {101} face. The values of kinetic coefficient of the steps, surface free energy, and ledge free energy of the step edge were determined.
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Key words:
- Protein crystallization /
- Impurity /
- Lysozyme
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