留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

微重力下胶原蛋白纤维化及羟基磷灰石结晶的初步研究

王静 蓝鼎 王育人 戴国亮 孔黎明

王静, 蓝鼎, 王育人, 戴国亮, 孔黎明. 微重力下胶原蛋白纤维化及羟基磷灰石结晶的初步研究[J]. 空间科学学报, 2015, 35(3): 330-335. doi: 10.11728/cjss2015.03.330
引用本文: 王静, 蓝鼎, 王育人, 戴国亮, 孔黎明. 微重力下胶原蛋白纤维化及羟基磷灰石结晶的初步研究[J]. 空间科学学报, 2015, 35(3): 330-335. doi: 10.11728/cjss2015.03.330
WANG Jing, LAN Ding, WANG Yuren, DAI Guoliang, KONG Liming. Preliminary Studies on Collagen Fibrosis and Hydroxyapatite Crystallization under Microgravity[J]. Journal of Space Science, 2015, 35(3): 330-335. doi: 10.11728/cjss2015.03.330
Citation: WANG Jing, LAN Ding, WANG Yuren, DAI Guoliang, KONG Liming. Preliminary Studies on Collagen Fibrosis and Hydroxyapatite Crystallization under Microgravity[J]. Journal of Space Science, 2015, 35(3): 330-335. doi: 10.11728/cjss2015.03.330

微重力下胶原蛋白纤维化及羟基磷灰石结晶的初步研究

doi: 10.11728/cjss2015.03.330
基金项目: 国家重点基础研究发展计划项目资助(2011CB710901)
详细信息
  • 中图分类号: V524

Preliminary Studies on Collagen Fibrosis and Hydroxyapatite Crystallization under Microgravity

  • 摘要: 在长期空间飞行过程中, 骨质丢失是一个严重问题. 羟基磷灰石(HAP)晶体是骨骼的主要成分, 骨骼中的胶原蛋白纤维在HAP生长结晶过程中起到关键作用. 研究了胶原蛋白纤维化过程在模拟微重力和常重力条件下的变化, 对以胶原 蛋白纤维作为模板生长出的HAP晶体形貌进行了观察. 结果表明, 不同浓度胶原蛋白溶液中形成的胶原蛋白纤维, 其内部孔隙数量和尺寸在模拟微重力条件下要明显大于常重力条件下, 胶原蛋白纤维内部孔隙的分布也不同于常重力条 件下的结果. 以模拟微重力条件下形成的胶原蛋白纤维为模板生长出的HAP 晶体主要为立方体状, 而以常重力条件下形成的胶原蛋白纤维为模板生长出的 HAP晶体形貌主要为板状. 该结果有助于未来进一步阐明空间骨质丢失的机理.

     

  • [1] Sun Lianwen, Zhuang Fengyuan. Research progress of microgravity induced osteopenia[J]. Chin. J. Aerospace Med., 2004, 15(1):54-58. In Chinese (孙联文, 庄逢源. 微重力导致航天员骨质疏松的研究进展[J]. 中华航空航天医学杂志, 2004, 15(1):54-58)
    [2] Stetlerstevenson W G, Veis A. Type-I collagen shows a specific binding-affinity for bovine dentin phosphophoryn[J]. Calcified Tissue Int., 1986, 38(3):135-141
    [3] Stetlerstevenson W G, Veis A. Bovine dentin phosphophoryn-calcium-ion binding-properties of a high-molecular-weight preparation[J]. Calcified Tissue Int., 1987, 40(2):97-102
    [4] Nudelman F, Lausch A J, Sommerdijk N A J M, Sone E D. In vitro models of collagen biomineralization[J]. J. Struc. Biol., 2013, 183:258-269
    [5] Nudelman F, Pieterse K, George A, Bomans P H H, et al. The role of collagen in bone apatite formation in the presence of hydroxyapatite nucleation inhibitors[J]. Nat. Mat., 2010, 9:1004-1009
    [6] Hulmes D J S, Wess T J, Prockop D J, Fratzl P. Radial packing, order, and disorder in collagen fibrils[J]. Biophys., 1995, 68(5):1661-1670
    [7] Traub W, Arad T, Weiner S. 3-dimensional ordered distribution of crystals in turkey tendon collagen-fibers[J]. Proc. Natl. Acad. Sci. USA, 1989, 86(24):9822-9826
    [8] Landis W J, Jacquet R. Association of calcium and phosphate ions with collagen in the mineralization of vertebrate tissues[J]. Calcified Tissue Int., 2013, 93(3):329-337
    [9] Cui Wei. Mechanism of bone mineral loss in microgravity[J]. Prog. Phys. Sci., 1998, 29(1): 84-86. In Chinese (崔伟. 微重力条件下骨矿盐丢失机理[J]. 生理科学进展, 1998, 29(1): 84-86)
    [10] John P, Karl E, Brass A. Simple physical model of collagen fibrillogenesis based on diffusion limited aggregation[J]. J. Mol. Biol., 1995, 247(4):823-831
    [11] John P, Karl E, Andy B. Self-assembly of rodlike particles in two dimensions: A simple model for collagen fibrillogenesis[J]. Phys. Rev., 1994, 50(4):2963-2966
    [12] Roedersheimer M T, Bateman T A, Simske S J. Effect of gravity and diffusion interface proximity on the morphology of collagen gels[J]. J. Biomed. Mater. Res., 1997, 37(2):276-281
    [13] Oyane A, Kim H M, Furuya T, et al. Preparation and assessment of revised simulated body fluids[J]. J. Biomed. Mater. Res.: Part A, 2003, 65A(2):188-195
    [14] Benedetto M, Chiara E G, Jake E B, Showan N N. Collagen gel fibrillar density dictates the extent of mineralization in vitro[J]. Soft Matter, 2011, 7(21):9898-9907
    [15] Oaki Y, Imai H. Experimental demonstration for the morphological evolution of crystals grown in gel media[J]. Cryst. Growth Des., 2003, 3(5):711-716
  • 加载中
计量
  • 文章访问数:  782
  • HTML全文浏览量:  12
  • PDF下载量:  1187
  • 被引次数: 0
出版历程
  • 收稿日期:  2014-06-18
  • 修回日期:  2015-01-04
  • 刊出日期:  2015-05-15

目录

    /

    返回文章
    返回