Effect of Substrate and Flow Rate on Hydroxyapatite Growthormalsize
-
摘要: 通过共混制备聚二甲基硅氧烷（PDMS）/羟基磷灰石（HAP）和PDMS/生物玻璃复合材料作为生长基板，研究了静止条件下不同基板上HAP的生长.设计了一套流速加载装置，观察模拟体液流速对生物玻璃基板上HAP晶体生长的影响.研究发现在静止条件下，HAP晶体在PDMS复合材料上比在生物玻璃上生长更快，尺寸更大；随着流速的增加，HAP晶体尺寸更大而且无定形沉淀数量减少.Abstract: Hydroxyapatite (HAP) has excellent biocompatibility and bioactivity. Synthetic HAP can be widely used as medical and eco-friendly materials. In order to know valuable information of study and development of bone tissue engineering, the influence on HAP crystals growth by the changed of fluid field and the chosen of substrates are studied. The composites materials are used as substrates, which are made of Polydimethylsiloxane (PDMS)/HAP, PDMS/bioglass, and pure bioglass, respectively, and the effect of different substrates on HAP growth are analyzed. The results show that HAP crystals growth rates are different on different substrates when the flow rate is zero. HAP crystals growth rate is highest on PDMS/HAP substrate. However, it is lowest on pure bioglass substrate. Moreover, the influence of different flow rates of HAP growth solution (Simulated Body Fluid, SBF) by a homemade flow rate adjustment apparatus is studied. Three different flow rates, 10ml/min, 15ml/min, and 30ml/min, are used in this study. Under SBF flow conditions, the sizes of HAP crystals increased and the amount of amorphous precipitate decreased.
-
Key words:
- Hydroxyapatite /
- Simulated body fluid /
- Flow rate /
- Substrate
-
[1] STAYTON P S, DROBNY G P, SHAW W J, et al. Molecular recognition at the protein-hydroxyapatite interface[J]. Crit. Rev. Oral Biol. M., 2003, 14(5):370-376 [2] MANN S. Biomineralization:Principles and concepts in bioinorganic materials chemistry[M]. London:Oxford University Press, 2001, 6-23 [3] MANN S, OZIN G A. Synthesis of inorganic materials with complex form[J]. Nature, 1996, 382(6859):313-318 [4] HEYWOOD B R, MANN S. Crystal recognition at inorganic-organic interfaces:nucleation and growth of oriented BaSO4 under compressed Langmuir monolayers[J]. Adv. Mater., 1992, 4(4):278-282 [5] MATSUMOTO T, OKAZAKI M, INOUE M, et al. Crystallinity and solubility characteristics of hydroxyapatite adsorbed amino acid[J]. Biomaterials, 2002, 23(10):2241-2247 [6] LU H B, MA C L, CUI H, et al. Controlled crystallization of calcium phosphate under stearic acid monolayers[J]. J. Cryst. Growth, 1995, 155(1-2):120-125 [7] REN Wei, CAO Xianying, FENG Lingyun, et al. Methods of synthetic nanometer hydroxyapatite and modification of its surface[J]. J. Chin. Ceramic Soc., 2001, 1:38-43(任卫, 曹献英, 冯凌云, 等. 纳米羟基磷灰石合成及表面改性的途径与方法[J]. 硅酸盐通报, 2001, 1:38-43) [8] REICH K M, GAY C V, FRANGOS J A. Fluid shear stress as a mediator of osteoblast cyclic adenosine monophosphate production[J]. J Cell Physio, 1990, 143(1):100-104 [9] BAKKER A D, SOEJIMAL K, KLEIN-NULEND J, et al. The production of nitric oxide and prostaglandin E2 by primary bone cells is shear stress dependent[J]. J. Biomech, 2001,34, 671-677 [10] MULLENDER M, EL HAJ A J, YANG Y, et al. Mechanotransduction of bone cells in fluid shear stress rate dependent[J]. Bicochem. Biophys. Res. Commun., 2004, 315(4):823-829 [11] CHEN N X, RYDER K D, PAVALKO F M, et al. Ca2+ regulates fluid shear-induced cytoskeletal reorganization and gene expression in osteoblasts[J]. Am. J. Physiol. Cell Physiol., 2000, 278(5):C989-997 [12] HUO B, KANG YY, HU M, et al. Advances of mechanical stimulation induced calcium response and transfer in osteoblasts[J]. J. Med. Biomech., 2011, 26(4):382-388. [13] BARAKAT A I, BARAKAT D K, GOJOVA A. secrets of the code:Do vascular endothelial cells use ion channels to decipher complex flow signal[J]. Biomaterials, 2006, 27(5):671-678 [14] DUAN Y R, ZHANG Z R, WANG C Y, et al. Dynamic study of calcium phosphate formation on porous HA/TCP ceramics[J]. J. Mater. Sci-Mater. M., 2005, 16, 795-801 [15] DENG Chunlin, CHEN Jiyong, FAN Hongsong, et al. Influence of Dynamic Flow Speed on Bonelike Apatite Formation in Porous Calcium Phosphate Ceramic in RSBF[J]. Key Eng. Mat. 2005, 288-289; 273-276 [16] OYANE A, KIM H M, FURUYA T, et al. Preparation and assessment of revised simulated body fluids[J]. J. Biomed. Mater. Res. A, 2002, 65A(2):188-195 -
-
计量
- 文章访问数: 1644
- HTML全文浏览量: 240
- PDF下载量: 2894
-
被引次数:
0(来源:Crossref)
0(来源:其他)
下载:
