Volume 35 Issue 3
May  2015
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WANG Jing, LAN Ding, WANG Yuren, DAI Guoliang, KONG Liming. Preliminary Studies on Collagen Fibrosis and Hydroxyapatite Crystallization under Microgravity[J]. Journal of Space Science, 2015, 35(3): 330-335. doi: 10.11728/cjss2015.03.330
Citation: WANG Jing, LAN Ding, WANG Yuren, DAI Guoliang, KONG Liming. Preliminary Studies on Collagen Fibrosis and Hydroxyapatite Crystallization under Microgravity[J]. Journal of Space Science, 2015, 35(3): 330-335. doi: 10.11728/cjss2015.03.330

Preliminary Studies on Collagen Fibrosis and Hydroxyapatite Crystallization under Microgravity

doi: 10.11728/cjss2015.03.330
  • Received Date: 2014-06-18
  • Rev Recd Date: 2015-01-04
  • Publish Date: 2015-05-15
  • Bone loss during long-term space flight is a serious problem. Collagen fibrils in bones are a key factor of Hydroxyapatite (HAP) crystal growth in its crystallization process, which is a main constituent of bones. In this paper, the changes of collagen fibrosis process under simulated microgravity and normal gravity conditions were studied, and the morphologies of HAP crystals grown on the surface of collagen fibrils were observed. Results showed that the amount and the size of pores inside collagen fibers formed under simulated microgravity condition and different concentrations of collagen were significantly larger than that under normal gravity condition and different concentrations of collagen. The distribution of the porosity under simulated microgravity condition was different with that under normal gravity condition. The morphology of HAP crystals under simulated microgravity condition was mainly cube-shaped. However, under normal gravity condition, HAP crystals were mainly plate-liked. These preliminary results will help to clarify the mechanism of bone loss in space in future.

     

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  • [1]
    Sun Lianwen, Zhuang Fengyuan. Research progress of microgravity induced osteopenia[J]. Chin. J. Aerospace Med., 2004, 15(1):54-58. In Chinese (孙联文, 庄逢源. 微重力导致航天员骨质疏松的研究进展[J]. 中华航空航天医学杂志, 2004, 15(1):54-58)
    [2]
    Stetlerstevenson W G, Veis A. Type-I collagen shows a specific binding-affinity for bovine dentin phosphophoryn[J]. Calcified Tissue Int., 1986, 38(3):135-141
    [3]
    Stetlerstevenson W G, Veis A. Bovine dentin phosphophoryn-calcium-ion binding-properties of a high-molecular-weight preparation[J]. Calcified Tissue Int., 1987, 40(2):97-102
    [4]
    Nudelman F, Lausch A J, Sommerdijk N A J M, Sone E D. In vitro models of collagen biomineralization[J]. J. Struc. Biol., 2013, 183:258-269
    [5]
    Nudelman F, Pieterse K, George A, Bomans P H H, et al. The role of collagen in bone apatite formation in the presence of hydroxyapatite nucleation inhibitors[J]. Nat. Mat., 2010, 9:1004-1009
    [6]
    Hulmes D J S, Wess T J, Prockop D J, Fratzl P. Radial packing, order, and disorder in collagen fibrils[J]. Biophys., 1995, 68(5):1661-1670
    [7]
    Traub W, Arad T, Weiner S. 3-dimensional ordered distribution of crystals in turkey tendon collagen-fibers[J]. Proc. Natl. Acad. Sci. USA, 1989, 86(24):9822-9826
    [8]
    Landis W J, Jacquet R. Association of calcium and phosphate ions with collagen in the mineralization of vertebrate tissues[J]. Calcified Tissue Int., 2013, 93(3):329-337
    [9]
    Cui Wei. Mechanism of bone mineral loss in microgravity[J]. Prog. Phys. Sci., 1998, 29(1): 84-86. In Chinese (崔伟. 微重力条件下骨矿盐丢失机理[J]. 生理科学进展, 1998, 29(1): 84-86)
    [10]
    John P, Karl E, Brass A. Simple physical model of collagen fibrillogenesis based on diffusion limited aggregation[J]. J. Mol. Biol., 1995, 247(4):823-831
    [11]
    John P, Karl E, Andy B. Self-assembly of rodlike particles in two dimensions: A simple model for collagen fibrillogenesis[J]. Phys. Rev., 1994, 50(4):2963-2966
    [12]
    Roedersheimer M T, Bateman T A, Simske S J. Effect of gravity and diffusion interface proximity on the morphology of collagen gels[J]. J. Biomed. Mater. Res., 1997, 37(2):276-281
    [13]
    Oyane A, Kim H M, Furuya T, et al. Preparation and assessment of revised simulated body fluids[J]. J. Biomed. Mater. Res.: Part A, 2003, 65A(2):188-195
    [14]
    Benedetto M, Chiara E G, Jake E B, Showan N N. Collagen gel fibrillar density dictates the extent of mineralization in vitro[J]. Soft Matter, 2011, 7(21):9898-9907
    [15]
    Oaki Y, Imai H. Experimental demonstration for the morphological evolution of crystals grown in gel media[J]. Cryst. Growth Des., 2003, 3(5):711-716
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