Volume 36 Issue 1
Jan.  2016
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YANG Xianjiong, MAO Xinjian, LUO Qing, SONG Guanbin. Wnt3a/β-catenin Signaling Pathway Mediates Inhibition of Proliferation of Mesenchymal Stem Cells Induced by Modeled Microgravity[J]. Journal of Space Science, 2016, 36(1): 63-69. doi: 10.11728/cjss2016.01.063
Citation: YANG Xianjiong, MAO Xinjian, LUO Qing, SONG Guanbin. Wnt3a/β-catenin Signaling Pathway Mediates Inhibition of Proliferation of Mesenchymal Stem Cells Induced by Modeled Microgravity[J]. Journal of Space Science, 2016, 36(1): 63-69. doi: 10.11728/cjss2016.01.063

Wnt3a/β-catenin Signaling Pathway Mediates Inhibition of Proliferation of Mesenchymal Stem Cells Induced by Modeled Microgravity

doi: 10.11728/cjss2016.01.063
  • Received Date: 2015-01-23
  • Rev Recd Date: 2015-07-02
  • Publish Date: 2016-01-15
  • Microgravity is known to play an important role in regulating the proliferation of Mesenchymal Stem Cells (MSC). However, information regarding the molecular mechanisms that link microgravity and MSC proliferation is still limited. In this study, using a parallel-plate clinostat to simulate microgravity effect, we examined the effect of Modeled Microgravity (MMG) on the proliferation of bone marrow-derived MSCs and the possible role of Wnt3a/β-catenin signaling pathway in this process. The results showed that MSCs subjected to MMG for 3 days exhibited significantly decrease of cell proliferation. Moreover, MMG weakened mRNA expression of Wnt3a, reduced unbound β-catenin in cytoplasm and subsequently inhibited the translocation of β-catenin into nucleus. Expression of Cyclin D1 at mRNA level in MSCs was also impaired after treatment with MMG. These results suggest that Wnt3a/β-catenin signaling pathway may be mediate inhibition of proliferation of MSCs induced by MMG.

     

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