Volume 37 Issue 2
Mar.  2017
Turn off MathJax
Article Contents
YUAN Junxia, YIN Hong, ZHAO Biao, ZHANG Lantao, QU Xi, XU Kanyan. Microbial Diversity Analysis in Spaceflight AIT Center[J]. Journal of Space Science, 2017, 37(2): 185-191. doi: 10.11728/cjss2017.02.185
Citation: YUAN Junxia, YIN Hong, ZHAO Biao, ZHANG Lantao, QU Xi, XU Kanyan. Microbial Diversity Analysis in Spaceflight AIT Center[J]. Journal of Space Science, 2017, 37(2): 185-191. doi: 10.11728/cjss2017.02.185

Microbial Diversity Analysis in Spaceflight AIT Center

doi: 10.11728/cjss2017.02.185
  • Received Date: 2015-12-11
  • Rev Recd Date: 2016-06-08
  • Publish Date: 2017-03-15
  • The assembly of spacecraft in clean room environment is essential for the prevention of forward contamination. In this study, both high-throughput sequencing and culture-based method are used to study the microbial community structure and diversity in the AIT (Assembly, Integration and Test) center of the spaceflight. The result of high-throughput sequencing analysis show that in the AIT center the dominant genus of airborne bacteria is Bacillus (78.47%±1.59%), and the dominant fungi is Tremellales (8.97%±0.93%). However, culture-based analysis indicates that the Staphylococcus is the dominant bacteria, and no fungal colony is observed in the AIT center. The microbial diversity is evaluated by chao1 index, Simpson index and Shannon index, and the results show that the bacterial diversity level is higher than that of fungal in the AIT center.

     

  • loading
  • [1]
    ZHANG L T, WEI C F, HOU Y Q, et al. Difficulties and countermeasures of microbe control in long-term manned spacecraft[J]. Life Sci. Instr., 2014(3):17-19(张兰涛, 魏传峰, 侯永青, 等. 长期载人航天器微生物控制难点及对策[J]. 生命科学仪器, 2014(3):17-19)
    [2]
    YAMAGUCHI N, ROBERTS M, CASTRO S, et al. Microbial monitoring of crewed habitats in space-current status and future perspectives[J]. Microbes Environ., 2014, 38(3). DOI: 10.1264/jsme1262.ME14031
    [3]
    ZHANG L T, WEI C F, BAI F L, et al. The microbial distribution of spaceflight AIT center[J]. Spacecraft Environ. Eng., 2014, 4(4):415-419(张兰涛, 魏传峰, 白梵露, 等. 载人航天器AIT中心微生物分布特征分析[J]. 航天器环境工程, 2014, 4(4):415-419)
    [4]
    VAISGAMPAYAN P A, RABBOW E, HORNECK G, et al. Survival of Bacillus pumilus spores for a prolonged period of time in real space conditions[J]. Astrobiology, 2012, 12(5). DOI: 10.1089/ast.2011.0738
    [5]
    GUO B, WU X L, QIAN Y. Approaches for increasing the culturability of microorganisms[J]. Acta Microbiol. Sin., 2006, 46(3):504-507(郭斌, 吴晓磊, 钱易. 提高微生物可培养性的方法和措施[J]. 微生物学报, 2006, 46(3):504-507)
    [6]
    LA DUC MT, VAISHAMPAYAN P, NILSSON HR, et al. Pyrosequencing-derived bacterial, archaeal, and fungal diversity of spacecraft hardware destined for Mars[J]. Appl. Environ. Microbiol., 2012, 78(16):5912. DOI: 5910.1128/AEM.01435-01412
    [7]
    VAISHAMPAYAN P, PROBST A J, DUC M T L, et al. New perspectives on viable microbial communities in low-biomass clean room environments[J]. ISME J., 2013, 7(2):312-324
    [8]
    CAPORASO J G, KUCZYNSKI J, STOMBAUGH J, et al. QIIME allows analysis of high-throughput community sequencing data[J]. Nature Methods, 2010, 7(5):335-336
    [9]
    HUGHES J B, HELLMANN J J, RICKETTS T H, et al. Counting the uncountable:statistical approaches to estimating microbial diversity[J]. Appl. Environ. Microbiol., 2001, 67(10):4399-4406
    [10]
    TAMURA K, STECHER G, PETERSON D, et al. MEGA6:Molecular Evolutionary Genetics Analysis version 6.0[J]. Molecular Biol. Evolution, 2013, 30:2725-2729
    [11]
    SCHWENDNER P, MOISSL-EICHINGER C, BARCZYK S, et al. Insights into the microbial diversity and bioburden in a south American spacecraft assembly clean room[J]. Astrobiology, 2013, 13(12):1140-1154.
    [12]
    MOELLER R, GUNTHER R, NICHILSON W L, et al. Mutagenesis in bacterial spores exposed to space and si-mulated Martian conditions:data from the EXPOSE-E spaceflight experiment PROTECT[J]. Astrobiology, 2012, 12(5). DOI: 10.1089/ast.2011.0739
    [13]
    LI F, YUAN H, ZHAO H. Review of microbiological control technologies in space and development proposals[J]. Manned Spaceflight, 2014, 5(5):465-473(李飞, 袁辉, 赵辉. 空间微生物控制技术综述[J]. 载人航天, 2014, 5(5):465-473)
    [14]
    MOISSL C, OSMAN S, DUC M T L, et al. Molecular bacterial community analysis of clean rooms where spacecraft are assembled[J]. FEMS Microbiol. Ecology, 2007, 61(3):509-521
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article Views(1235) PDF Downloads(1141) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return