Abstract: We designed and manufactured a biostack, monolayers of Artemia Salina eggs sandwiched between nuclear emulsions, to investigate the biological effect of single heavy ions to individual cells. It was exposed on board "8885" Chinese Satellite during eight-day flight and then after it returned to the ground it was collected. The distribution of tracks left by space heavy ions on emulsion was studed by means of image analysis instrument. And the development of Artemia Salina eggs was observed. Comparing with control group on ground, we found that the group on board have lower hatching rate and delayed developing time. Out of 90 experimental eggs only 8 eggs showed development at different levels. Among them 4 hatching eggs were not hit by the heavy ion and among the other 4 emerging eggs there were 3 eggs hit. Under the same condition hatching rate of control group on ground reached at almost 60%. These phenomena are obviously related to the action of heavy ions in space.From the results of the test we reached the following conclusion: the biosample plate-nuclear emulsion shadow location technique can give information of Artemia Salina eggs hit by heavy ions clearly and exactly. That is one of thte experimental and analysing methods useful to investigate biological effects and mechanism on heavy ion action.