Abstract: The static calibration test system for Lorentz Actuators of active payload-level vibration isolation system for experiments in space is systematically studied by simulations and tests. In order to meet the demand for real-time changes controller outputs in high quality microgravity vibration isolation system, actuator design parameters should be inspected and the relationship between output force and the input current should be deduced. A test system was designed, and its hardware structure, software model as well as the test program diagram, were introduced. The system hardware contains force sensors, force value shows, three axis displacement control apparatus, motor drive module and data receiving and distribution module. The LabVIEW programming technology was utilized in the system software to realize signal sampling, data displaying and storage. Finally, Matlab was used to process data. The test results provide technical basis for further optimal design of the actuators. By fitting error graphics, the correctness and rationality of the system design were verified, which provides a reliable guarantee for the normal operation of the active vibration isolation system.