Wnt3a/β-catenin信号通路调节模拟微重力诱导的骨髓间充质干 细胞增殖抑制
doi: 10.11728/cjss2016.01.063 cstr: 32142.14.cjss2016.01.063
Wnt3a/β-catenin Signaling Pathway Mediates Inhibition of Proliferation of Mesenchymal Stem Cells Induced by Modeled Microgravity
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摘要: 微重力环境对骨髓间充质干细胞(MSCs)的增殖行为起着重要调节作用, 但其 中的分子机理尚不清楚. 采用平行平板旋转培养装置模拟微重力效应, 考察了 模拟微重力效应下MSCs增殖行为的变化以及Wnt3a/β-catenin信号通路在 该过程中的作用. 结果发现, 模拟微重力效应明显抑制MSCs的增殖行为, 下调 Wnt3a mRNA的表达, 降低细胞质中游离β-catenin, 减少β-catenin 向细胞核转移, 抑制Cyclin D1的表达. 结果表明, Wnt3a/β-catenin信 号通路可能介导了微重力效应诱导的MSCs增殖抑制.Abstract: Microgravity is known to play an important role in regulating the proliferation of Mesenchymal Stem Cells (MSC). However, information regarding the molecular mechanisms that link microgravity and MSC proliferation is still limited. In this study, using a parallel-plate clinostat to simulate microgravity effect, we examined the effect of Modeled Microgravity (MMG) on the proliferation of bone marrow-derived MSCs and the possible role of Wnt3a/β-catenin signaling pathway in this process. The results showed that MSCs subjected to MMG for 3 days exhibited significantly decrease of cell proliferation. Moreover, MMG weakened mRNA expression of Wnt3a, reduced unbound β-catenin in cytoplasm and subsequently inhibited the translocation of β-catenin into nucleus. Expression of Cyclin D1 at mRNA level in MSCs was also impaired after treatment with MMG. These results suggest that Wnt3a/β-catenin signaling pathway may be mediate inhibition of proliferation of MSCs induced by MMG.
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Key words:
- Microgravity /
- Mesenchymal stem cell /
- Cell proliferation /
- Wnt signal
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