基于超高分辨率和超长深度光学相干层析成像技术的悬尾鼠航天相关神经眼综合征研究

陈思思; 张玺; 郑谷; 汪庆映; 丁学雯; 陈宇雷; 舒咬根

doi:10.11728/cjss2023.05.2023-05-yg11

基于超高分辨率和超长深度光学相干层析成像技术的悬尾鼠航天相关神经眼综合征研究

doi: 10.11728/cjss2023.05.2023-05-yg11 cstr: 32142.14.cjss2023.05.2023-05-yg11

陈思思^{1, 2, 3, 4,},
张玺²,
郑谷²,
汪庆映²,
丁学雯²,
陈宇雷²,
舒咬根^{1, 3, 4, ,}

1.
国科温州研究院温州生物材料与工程研究所　温州　325001
2.
温州医科大学附属眼视光学院　温州　325027
3.
国家药监局疫苗及生物制品质量监测与评价重点实验室　温州　325001
4.
瓯江实验室（再生调控与眼脑健康浙江省实验室）　温州　325024

基金项目: 国科温州研究院启动基金项目（wiucasqd2020009）和瓯江实验室高端医疗器械集群启动基金项目（OJQDJQ2022001）共同资助

详细信息

作者简介:

陈思思：E-mail：chensisi@ucas.ac.cn

通讯作者:

舒咬根，E-mail：shuyaogen@ucas.ac.cn

中图分类号: V524
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- 文章访问数: 257
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出版历程
- 收稿日期: 2023-08-07
- 修回日期: 2023-09-01
- 网络出版日期: 2023-10-11

Study on Spaceflight-associated Neuro-ocular Syndrome with the Rat Tail Suspension by ULOCT and UHROCT

CHEN Sisi^{1, 2, 3, 4
,},
ZHANG Xi²,
ZHENG Gu²,
WANG Qingying²,
DING Xuewen²,
CHEN Yulei²,
SHU Yaogen^{1, 3, 4
, ,}

1.
Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325001
2.
Eye Hospital and School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou 325027
3.
Research Center of Quantitative Detection Technology, NMPA Key Laboratory for Quality Monitoring and Evaluation of Vaccines and Biological Products, Wenzhou 325001
4.
Oujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), Wenzhou 325024

摘要

摘要: 为研究航天相关神经眼综合征（SANS）相关特征参数与微重力的相关性，以Sprague-Dawley（SD）大鼠为悬尾动物模型，构建了超长扫描深度的光学相干断层扫描（ULOCT）装置，测量大鼠眼部特征参数（角膜、前房、晶状体厚度和玻璃体腔深度及眼轴长度），以及视网膜超高分辨率（UHROCT）测量装置，测量大鼠眼底亚层结构参数（视神经层、视网膜内层、视网膜外层、脉络膜和巩膜的厚度）。结果表明大鼠悬尾1月后，眼底彩照未见明显视盘水肿等症状，但OCT结果显示大鼠眼结构发生明显改变：角膜变薄（p<0.01），眼轴缩短（p<0.05），视网膜外层变薄（p<0.01）。这些特征参数变化（尤其是眼轴明显缩短）展现了SANS亚临床前期的症状。本文为后续微观层面的SANS分子机制研究提供了宏观动物模型以及在体眼部测量新方法。
- 微重力 /
- 航天相关神经眼综合征 /
- 光学相干断层成像 /
- 眼轴缩短
Abstract: To investigate the correlation between the relevant characteristic parameters of Spaceflight-Associated Neuro-ocular Syndrome (SANS) and microgravity, Sprague-Dawley (SD) rats with a tail-suspended animal model were utilized. Ultra-Long Scan Depth Optical Coherence Tomography (ULOCT) system was set up to measure the characteristic parameters of the rat eyes (corneal thickness, anterior chamber depth, lens thickness, vitreous chamber depth, and axial length), and an Ultra-High-Resolution Optical Coherence Tomography (UHROCT) system to measure the sublayer structural parameters of the rat fundus (retinal nerve layer, inner retinal layer, outer retinal layer, choroid, and sclera thickness). The results came from the fundus photographs showed that there were no obvious symptoms of optic disc edema. However, the results came from ULOCT and UHROCT showed the significant changes in the rat eye structure: corneal thinning (p<0.01), axial shortening (p<0.05), and outer retinal thinning (p<0.01). These changes of characteristic parameters, especially the significant axial shortening, exhibited subclinical symptoms of SANS. This study provides a macroscopic animal model and a novel in vivo ocular measurement method for investigating the molecular mechanisms of SANS at the microscopic level in future researches.
- Microgravity /
- Spaceflight Associated Neuro-Ocular Syndrome (SANS) /
- Optical Coherence Tomography (OCT) /
- Shortening of axial length

HTML全文

图 1 大鼠悬尾模型实验

Figure 1. Diagram of rat tail suspension

下载: 全尺寸图片幻灯片

图 2 大鼠眼成像及分析。（a）大鼠眼底彩照，OD表示右眼，（b）大鼠超长深度OCT全眼成像，（c）大鼠超高分辨率OCT眼底成像及分层界限和层次定义。白色竖线为200 μm标尺

Figure 2. Schematic representation of rat ocular imaging and analysis. (a) Rat fundus color photograph (OD represents the right eye), (b) rat ultra-long depth OCT imaging of the entire eye, (c) rat high-resolution OCT fundus imaging with layer boundaries and definitions, with the white vertical line representing a 200 μm scale

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图 3 大鼠体重随悬尾天数的变化统计（统计数量n=6。数据显示第18天后二者的体重无统计差异）

Figure 3. Statistical rat body weight over the duration of tail suspension（The data indicated that there were no statistically significant differences in body weight between the two groups after the 18th day, the number of statistical samples for each group n is equal to 6）

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图 4 大鼠眼部特征参数（a）及眼底亚层结构参数统计分析（b）。数据表明悬尾一个月后，眼轴明显缩短且视网膜外层厚度显著下降

Figure 4. Statistical graph of rat ocular feature parameters (a) and sublayer parameters of the fundus (b). The data indicated that after suspension of one month there was a significant decrease in axial length and outer retina thickness

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