白藜芦醇对后肢去负荷雄性大鼠生殖 损伤的对抗作用
doi: 10.11728/cjss2024.01.2023-0063 cstr: 32142.14.cjss2024.01.2023-0063
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轩莹莹：女, 1998年12月出生于河南省周口市, 现为兰州大学药学院硕士在读研究生, 主要研究方向为空间骨流失机制与防治措施. E-mail: xuanyingy@163.com
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通讯作者:
Antagonistic Effects of Resveratrol on Reproductive Injury in Hind-limp Unloading Male Rats
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摘要: 为 研究白藜芦醇对后肢去负荷雄性大鼠生殖损伤的对抗作用, 将30只实验雄性大鼠随机分为安慰剂组(placebo control, PC)、后肢去负荷+安慰剂组(hind-limp unloading + placebo control, HU+PC)、后肢去负荷+白藜芦醇给药组(hind-limp unloading + Resveratrol, HU+Res). HU+Res组按隔天30 mg·kg–1的剂量腹腔注射, PC组和HU+Res组腹腔注射等体积生理盐水. 28 d后处死所有雄性大鼠, 取睾丸和附睾, 用于组织病理学检测、组织生化指标检测、组织蛋白表达分析. 与PC组相比, HU+PC组雄性大鼠睾丸和附睾重量均显著下降, 白藜芦醇可以显著改善这些指标; 组织病理学结果显示, HU+PC组雄性大鼠睾丸组织生精上皮层数显著减少, 细胞间质面积显著增大, 出现明显的水肿, 精原细胞数目明显减少, 经白藜芦醇处理后, 睾丸组织形态有部分恢复; 氧化应激与炎症因子水平检测结果显示, 与PC组相比, HU+PC组雄性大鼠睾丸组织氧化应激水平失衡, 炎症因子水平明显升高, 经白藜芦醇处理后, 可以明显逆转这一有害情况; Western blot 结果显示, 白藜芦醇能显著降低HU+PC组大鼠睾丸组织Bax表达水平, 提高Bcl-2, p-PI3K/PI3K, p-AKT/AKT表达水平, 表明30 mg·kg–1的白藜芦醇可能通过激活PI3K/AKT信号通路发挥对后肢去负荷雄性大鼠生殖损伤的对抗作用.Abstract: To research the antagonistic effect of resveratrol on reproductive injury in hind-limp unloading male rats. The 30 experimental rats were randomly divided into placebo control group, hind-limp unloading group, and HU+Res group. The HU+Res group was given resveratrol at the dose of 30 mg·kg–1 respectively every two days, and the placebo control group and hind-limp unloading groups were given equal volume of physiological saline. After 28 days, all rats were executed, and testes and epididymis were taken for histopathology detection, tissue biochemical index detection, and protein expression analysis of testicular tissue. Compared with the placebo control group, the weight of testes and epididymis in the hind-limp unloading group was significantly reduced, and resveratrol could significantly improve these indicators. The results of histopathology showed that the number of seminiferous epithelium layers and the area of intercellular substance in the hind-limp unloading group were significantly reduced, with obvious edema, and the number of spermatogonium was significantly reduced. After treatment with resveratrol, the morphology of testicular tissue was partially restored. The detection results of oxidative stress and inflammatory factor levels showed that compared with the placebo control group, the hind-limp unloading group showed an imbalance in oxidative stress levels in the testicular tissue of rats, with a significant increase in inflammatory factor levels. After treatment with resveratrol, this harmful situation could be significantly reversed. Western blot results showed that resveratrol significantly reduced the expression levels of Bax in the testicular tissue of hind-limp unloading male rats, and increased the expression levels of Bcl-2, p-PI3K/PI3K, p-AKT/AKT. Conclusions resveratrol of 30 mg·kg–1 may exert a antagonistic effect on reproductive damage in hind-limp unloading male rats by activating the PI3K/AKT signaling pathway.
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Key words:
- Resveratrol /
- Male rat /
- Reproduction /
- Tail suspension
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图 1 各组大鼠睾丸形态、睾丸系数和附睾系数. (a)睾丸外观, (b)睾丸直径, (c)睾丸横径, (d)睾丸系数, (e)附睾系数
Figure 1. Appearance of testis and the quality of testis and epididymis in each group. (a) Appearance of testis, (b) major diameter of testis, (c) transverse diameter of testis, (d) quality of testis, (e) quality of epididymis
图 3 各组大鼠附睾精子数量 (a) 和精子畸形率 (b)
Figure 3. Sperm count (a) and sperm abnormality rate (b) in each group
图 4 每组大鼠睾丸组织中SOD(a), CAT(b), GSH-PX(c), H2O2(d), LDH(e)和MDA(f)的含量/酶活力水平
Figure 4. Content or activity of SOD (a), CAT (b), GSH-PX (c), H2O2 (d), LDH (e) and MDA (f) in each group
图 5 各组大鼠睾丸组织炎症因子IL-6(a), IL-13(b)和IL-1β(c)水平
Figure 5. Content of IL-6 (a), IL-13 (b) and IL-1β (c) in each group
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